Are you looking for one night in Amsterdam? If so, you could probably save money by booking a layover of under 24 hours on one ticket. If you go over 24 hours it will be considered a stop and the price goes up. You don't mention your airline, but I am assuming KLM and partners. There is an 8:00 flight to Detroit on Northwest that would give you 23:50 in AMS and count as a layover.
It can be difficult to book this online as most booking engines don't like to book overnight layovers. Orbitz will often do it if you use the departure time to force the overnight connection, but it might be tough with the early arrival in AMS. Might be a good time to pay the $10 booking fee to do it on the phone.
If you are on separate tickets you the risk of getting the shaft at AMS if you flight is delayed.
You won't be protected on that connection. If your PRG-AMS flight is on time I'd say you are fine.
I completly misread the OP thinking it was an overnight. Ignore my post above, unless you do decide to spend a day in AMS.
We missed our plane back to Detroit last year. We had an overnight layover so had to check our bags before our flight home. We got to the Schipol airport 3 hours early and due to the bombing in Glasgow, didn't make it though security in time to get to our gate.
Not that this will happen to you, but its always a possibility.
Now that my coffee has kicked in and I can think, there should be no need to check luggage in AMS. Again I'm making an assumption you are flying KLM, Czech, NW, Continental or Delta - all of whom are members of Skyteam and can interline your luggage. It looks like both KLM and Czech operate AMS-PRG, but there is no need to claim your luggage in AMS regardless of which you fly. Just make sure they check it through to your final destination. You will have to claim it for customs at your first stop in the US. They should even be able to print your boarding pass for your US bound flight in PRG - otherwise just stop at a transfer desk. 2.5 hours is plenty of time in AMS.
If you're checking bags, the problem is that you'll have to get your bags and then go to check-in. On this side of the pond, they usually want international bags two hours before departure and I don't see how you could make that.
Any chance you can book a later flight from Schipol???
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