If you get off at San Marco, and then take another one returning, that would be okay, otherwise you will get a fine for extending the journey.
See the official rule:"
"7,00 € - WATER SERVICES TICKET, 60’
Allows travel on all services (except for those of routes Alilaguna, and Actv routes no. 16, 19, 21 and Casinò) for 60 minutes from stamping, with transport means change allowed in the same direction. Does not include the return journey. Included in the price is the transport of one luggage item of up to 150 cm as the sum of its three dimensions. Can be purchased from the Hellovenezia ticket desks and the authorized resellers"
The line one does not turn round at Sn Marco it terminates at the Lido,
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