This was actually the first art gallery we saw while in Italy. It was very nice. It's less crowded than I would have thought but very interesting. It's also relatively inexpensive to get in.. I thing it was only 5 Euro each.
A downside to the place is no audio guides are available, but fortunately they have information cards in each room that you can pick up that describe the more significant paintings and fresco cellings. We spent about 2.5hrs in here.
You do need to check your bags before entering, and no photos are allowed of course. The worst part I found was Rome is so hot you always feel like you need some water, but it's not allowed inside the gallery so drink up before entering.
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