Can't say I did the Segway Tour... However, I did do the Electric Bike Tour ran by the same people. FANTASTIC! I loved it and more importantly my mum even loved it. She was extremely nervous before she left but came back and said that it was the highlight of her trip.
There is a choice of a few different routes (take the blue route!). The route took us round all of the main attractions including the Castle, Charles Bridge, Old Town, New Town and Jewish Quarter.
The tour guide was fantastic. Sometimes I find that tour guides often tell us too much - but our tour guide told us all the interesting details quickly so we could get on and enjoy the electric bikes.
Electric bikes are extremely easy to use, even if you're not a budding cyclist!
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